Micromolding-based encapsulation of mesenchymal stromal cells in alginate for intraarticular injection in osteoarthritis - Institut Galilée Université Paris 13 Accéder directement au contenu
Article Dans Une Revue Materials Today Bio Année : 2023

Micromolding-based encapsulation of mesenchymal stromal cells in alginate for intraarticular injection in osteoarthritis

Julie Baron
  • Fonction : Auteur
Joelle Veziers
  • Fonction : Auteur

Résumé

Osteoarthritis (OA) is an inflammatory joint disease that affects cartilage, subchondral bone, and joint tissues. Undifferentiated Mesenchymal Stromal Cells are a promising therapeutic option for OA due to their ability to release anti-inflammatory, immuno-modulatory, and pro-regenerative factors. They can be embedded in hydro-gels to prevent their tissue engraftment and subsequent differentiation. In this study, human adipose stromal cells are successfully encapsulated in alginate microgels via a micromolding method. Microencapsulated cells retain their in vitro metabolic activity and bioactivity and can sense and respond to inflammatory stimuli, including synovial fluids from OA patients. After intra-articular injection in a rabbit model of post-traumatic OA, a single dose of microencapsulated human cells exhibit properties matching those of non-encapsulated cells. At 6 and 12 weeks post-injection, we evidenced a tendency toward a decreased OA severity, an increased expression of aggrecan, and a reduced expression of aggrecanase-generated catabolic neoepitope. Thus, these findings establish the feasibility, safety, and efficacy of injecting cells encapsulated in microgels, opening the door to a long-term follow-up in canine OA patients.
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hal-04072260 , version 1 (04-06-2024)

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Fabien Nativel, Audrey Smith, Jeremy Boulestreau, Charles Lépine, Julie Baron, et al.. Micromolding-based encapsulation of mesenchymal stromal cells in alginate for intraarticular injection in osteoarthritis. Materials Today Bio, 2023, 19, pp.100581. ⟨10.1016/j.mtbio.2023.100581⟩. ⟨hal-04072260⟩
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